Detected by staining with 0.1%
bromocresol green or 1:1 mixture of bromocresol green+0.5% orange G solution after acid digestion of the flour particles. In contrast to
the standard AACC method of staining with iodine solution where both eggs and flour particles were stained brownish-yellow or yellow,
bromocresol green alone or in mixture with orange G, differentially stained the insect eggs and the flour particles distinct orange and
green, respectively.
كود: تحديد الكل
Detected by staining with 0.1%
bromocresol green or 1:1 mixture of bromocresol green+0.5% orange G solution after acid digestion of the flour particles. In contrast to
the standard AACC method of staining with iodine solution where both eggs and flour particles were stained brownish-yellow or yellow,
bromocresol green alone or in mixture with orange G, differentially stained the insect eggs and the flour particles distinct orange and
green, respectively.
منقول من بحث علمى من abstract
رابط التحميل
https://www.researchgate.net/publication/235247751_Detection_of_eggs_of_stored-product_insects_in_flour_by_staining_technique
~~~~~~~~~
كان يوجد طريقة مقدمة من AACC
ولكن فى هذا البحث يصف انها كانت غير فعالة فى عملية تمييز بيض الحشرات من جزيئات الدقيق فى عملية التصبغ كلهم لون pink فلايمكن التفرقة بينهم
اما فى هذا البحث يصبغ بيض الحشرات orange والدقيق green .
~~~~~~~~~~~~~~~
بعض طرق الكشف عن بعض انواع من بيض الحشرات فى الدقيق باستخدام الصبغات كما فى البحث المرفق
There are staining methods to detect insect (Sitophilus spp.) egg plugs in whole grains using :-
acid fuchsin (0.05%)
gentian violet and berberine sulphate (20 ppm) (Rajendran,2005).
~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~
In the recommended American Associa-
tion of Cereal Chemists (AACC) (2000) method
1- white flour or refined wheat flour containing eggs is sifted using
an 80–100 mesh sieve(177-149 micron)
2- the flour residues digested with
sulphuric acid.
3- Subsequently, the eggs are stained with0.1 N iodine solution and turn yellow. ~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~
Blumberg and
Ballard (1941) reported an acid–alkali method involving :-
treatment with 5% nitric acid followed by 10% sodium hydroxide in which the eggs were orange-coloured even without the addition of any stain.
~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~
In preliminary tests, various stains and dyes (total 57)
were evaluated for staining insect eggs .
Based on the results,
bromocresol green (tetrabromo-m cresolsul-fonphthalein),
orange G (Acid Orange 10)
and acid fuchsin
(Acid Violet 19) were chosen for the final tests.
The AACC
(2000) method with certain modifications was adopted for staining the eggs. Instead of the iodine solution, bromo-cresol green, a 1:1 mixture of bromocresol green+orange
G and acid fuchsin were used for the staining and the duration of staining was 2–3 min. Also, for the final washing of flour particles and eggs, 10% sulphuric acid solution was used instead of 1% sulphuric acid.
Bromo-cresol green (0.1%) in 100 ml of water containing 1.45 ml of
0.1 N sodium hydroxide and aqueous solutions of acid fuchsin (0.05%), orange G (0.5%), and iodine (0.1 N) were
prepared.
كود: تحديد الكل
There are staining methods to detect insect (Sitophilus spp.) egg plugs in whole grains using :-
acid fuchsin (0.05%)
gentian violet and berberine sulphate (20 ppm) (Rajendran,2005).
~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~
In the recommended American Associa-
tion of Cereal Chemists (AACC) (2000) method
1- white flour or refined wheat flour containing eggs is sifted using
an 80–100 mesh sieve(177-149 micron)
2- the flour residues digested with
sulphuric acid.
3- Subsequently, the eggs are stained with0.1 N iodine solution and turn yellow. ~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~
Blumberg and
Ballard (1941) reported an acid–alkali method involving :-
treatment with 5% nitric acid followed by 10% sodium hydroxide in which the eggs were orange-coloured even without the addition of any stain.
~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~•~
In preliminary tests, various stains and dyes (total 57)
were evaluated for staining insect eggs .
Based on the results,
bromocresol green (tetrabromo-m cresolsul-fonphthalein),
orange G (Acid Orange 10)
and acid fuchsin
(Acid Violet 19) were chosen for the final tests.
The AACC
(2000) method with certain modifications was adopted for staining the eggs. Instead of the iodine solution, bromo-cresol green, a 1:1 mixture of bromocresol green+orange
G and acid fuchsin were used for the staining and the duration of staining was 2–3 min. Also, for the final washing of flour particles and eggs, 10% sulphuric acid solution was used instead of 1% sulphuric acid.
Bromo-cresol green (0.1%) in 100 ml of water containing 1.45 ml of
0.1 N sodium hydroxide and aqueous solutions of acid fuchsin (0.05%), orange G (0.5%), and iodine (0.1 N) were
prepared.
بالتوفيق